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216463

DMP1 (DMP-1, Dentin Matrix Protein 1)

Size

£704.00

SKU:
216463
Purity:
Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Recombinant protein corresponding to human DMP-1.
Clone:
14L367
Format:
Supplied as a lyophilized powder in PBS. Reconstitute with 500ul of sterile PBS.
Extra Details:
DMP-1(dentin matrix protein1) is an acidic,100-160kD member of the phosphophoryn family of extracellular matrix proteins. Mouse DMP-1 is found both intra- and extracellularly, where phosphorylated DMP-1 participates in bone mineralization. Secreted mature mouse DMP-1 is a 487aa proteoglycan. It contains multiple serine, glutamic acid, and aspartic acid residues, a GAG attachment site (aa71-72) and an RGD motif (aa350-352). Multiple cleavage sites exist between aa’s205-253, creating multiple fragments approximately 37kD and 57kD in size. Mature mouse DMP-1 is 87% and 63% aa identical to rat and human DMP-1, respectively. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice