Complement Component 5a (C5a)

Size

£691.00

SKU:: 216370
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Complement Component C5/C5a.
Clone:: 14L274
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul of sterile PBS.
Extra Details:: C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Applications: Suitable for use in ELISA, Western Blot, Neutralization. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:2000 Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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