Common beta chain

Size

£704.00

SKU:: 216367
Application:: WB
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Common beta chain
Clone:: 14L271
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Extra Details:: The receptors for human granulocyte macrophage-colony stimulating factor (GM-CSF), IL-3 and IL-5 are comprised of a cytokine-specific alpha chain, and a common beta chain. The common beta chain on GM-CSF, IL-3, and IL-5 receptors interacts with all three ligands, promoting some degree of overlap between their regulation of hematopoietic cell signaling. GM-CSF is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts, in response to cytokine or immune and inflammatory stimuli. IL-3, also known as mast cell growth factor, is a pleiotropic factor produced primarily by activated T cells. IL-3 can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells, as well as various lineage committed progenitors. IL-5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 is also a growth and differentiation factor for B cells. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice