CD80 (B7-1)

Size

£704.00

SKU:: 216333
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: Recombinant protein corresponding to mouse CD80.
Clone:: 14L237
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 400ul sterile PBS.
Extra Details:: CD80 is a 60kD highly glycosylated protein. It is a member of the Ig superfamily, also known as B7-1, B7 and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:1000 Immunohistochemistry: 1:100-1:300 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice