CD45R (B220)

Size

£704.00

SKU:: 216310
Purity:: Purified by Protein G affinity chromatography from culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Leukemia virus-induced pre-B tumor cells.
Clone:: 14L214
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Extra Details:: CD45R is an isoform of CD45, also known as B220. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:100-1:400 Immunohistochemistry (Paraffin): 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice