CD40 Ligand (CD154, CD40-L)

Size

£704.00

SKU:: 216307
Purity:: Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: Mouse Th1 cells.
Clone:: 14L211
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 400ul sterile PBS.
Extra Details:: Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function. CD154 has been shown to be hyperexpressed by B and T cells in SLE patients. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:300 Immunohistochemistry: 1:100-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.