CD314 (NKG2D, Natural Killer Group 2, Member D)

Size

£889.00

SKU:: 216290
Application:: Flow Cytometry
Purity:: Purified by Protein A/G affinity chromatography from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: Soluble mouse NKG2D
Clone:: 14L194
Format:: Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with sterile PBS.
Extra Details:: NKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells, a subset of CD8+ T cells, g/d T cells and NK1.1+ T cells, as well as in vitro induced LAK cells. NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans. present in both mice and humans. NKG2D ligands trigger cytokine (IFN-g, GM-CSF, TNF-a, MIP1b and others) and granzyme release from NK cells. Applications: Suitable for use in Flow Cytometry and Blocking. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin: <0.002EU/ug Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice