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216108

Activin Receptor Type IIA (Activin RIIA)

Size

£704.00

SKU:
216108
Purity:
Purified by Protein G affinity chromatography from culture supernatant.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Recombinant protein corresponding to human Activin RIIA
Clone:
14L12
Format:
Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
Extra Details:
Activin isoforms and other members of the TGF­beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine­threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR­I(A), ActR­IB, ActR­II(A) and ActR­IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR­IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high­affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor­activin complex and initiate signal transduction. Besides the activin isoforms, ActR­II will also bind inhibin, BMP­2 and BMP­7 with lower affinities. ActR­I can also bind and form signaling complexes with the BMP­2/7­bound BMPR­II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1000 Immunohistochemistry: 1:50-100 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice