Interleukin-6 (IL-6, B-Cell Stimulatory Factor-2, BSF-2)

Size

£812.00

SKU:: 216017
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Interleukin-6.
Clone:: 8E10
Format:: Supplied as a lyophilized powder in 0.01M PBS, pH 7.0, 1mg/ml BSA, 0.09% sodium azide. Reconstitute with ddH2O.
Extra Details:: IL-6, also called B-cell stimulatory factor-2 (BSF-2), is an important cytokine for the differentiation of B-cells into immunoglobulin-secreting cells. IL-6 is mainly produced by T-cells, macrophages and endothelial cells in response to pathogenic molecular stimuli during infection, trauma and the growth phase of some types of cancer. IL-6 is up-regulated by the binding of Toll-like receptors to antigen pattern ligands. In acute inflammatory phase, IL-6 causes increased body temperature by initiating Prostaglandin E2 synthesis in hypothalamus. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice