Antichymotrypsin, alpha 1 (a1-ACT)

Size

£748.00

SKU:: 215976
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human plasma derived alpha1-antichymotrypsin.
Clone:: 14J2
Format:: Supplied as a lyophilized powder in 0.01M PBS, pH 7.2. Reconstitute with ddH2O.
Extra Details:: alpha1-antichymotrypsin (a1-ACT) presents in plasma at an average concentration of 0.45mg/ml. It is a protease inhibitor for chymotrypsin-like serine proteinases, such as cathepsin G in neutrophils and chymases in mast cells. The complex formed by a1-antichymotrypsin and prostate specific antigen (a1-ACT-PSA) has been used as a biomarker for prostate cancer. a1-ACT is produced in liver and is increased during acute phase reaction. Deficiency of this protein can cause liver disease and chronic obstructive pulmonary disease. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.