Vascular Endothelial Growth Factor (VEGF-A, Vascular Permeability Factor, VPF, VEGFA, VEGF)

Size

£606.00

SKU:: 215509
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa33-48, VDIFQEYPDEIEYIFK, sequence at the N-terminal of human Vascular Endothelial Growth Factor. Species Sequence Homology: mouse.
Format:: Supplied as a lyophilized powder from PBS 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Vascular endothelial growth factor (VEGF) is a disulfide linked homodimeric protein associated with angiogenesis, vasculogenesis and endothelial cell growth. 10 isoforms of VEGF are produced by alternative splicing with varying levels of expression. The isoforms VEGF189, VEGF165 and VEGF121 are widely expressed whereas the isoforms VEGF206 and VEGF145 are uncommon. Isoforms VEGF165 and VEGF121 are freely secreted whereas isoform VEGF189 remains cell associated. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.5ug/ml Immunohistochemistry (Frozen, FFPE): 0.5-1ug/ml. Heat mediated antigen retrieval is required for FFPE. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice