Ubiquitin C Terminal Hydrolase 1(Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1, UCH-L1, Neuron Cytoplasmic Protein 9.5, PGP 9.5, PGP9.5, Ubiquitin Thioesterase L1, UCHL1)

Size

£735.00

SKU:: 215505
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Recombinant full length protein corresponding to human Ubiquitin C-terminal Hydrolase 1, expressed in E. coli.
Format:: Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Extra Details:: This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:10,000, detects ~24kD UCHL1 enzyme Immunocytochemistry: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Rat spinal cord or peripheral nerve homogenate Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.