SPTAN1 (Non-Erythrocyte Alpha-Spectrin, Alpha II Spectrin, Alpha Fodrin, Spectrin, Non-erythroid Alpha Chain, SPTA2)

Size

£735.00

SKU:: 215470
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to the seventh, eight and ninth repeats aa676-1043 of human alpha-II Spectrin, expressed in E. coli. The 9th spectrin repeat also includes a Src-homology 3 domain.
Clone:: 3D7
Format:: Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Extra Details:: Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. The Spectrins form into dimers and further into tetramers of alpha and beta subunits (Ref: Entrez Gene). The alpha-II subunit is widely expressed in tissues but, in the nervous system, is found predominantly in neurons. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:500-1:1000 Western Blot: 1:5000-1:10,000 Immunohistochemistry: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice