Neuron Specific Enolase (Gamma-Enolase, EC 4.2.1.11, 2-phospho-D-glycerate Hydrolyase, Neural Enolase, Neuron-specific Enolase, NSE, Enolase 2, ENO2) (Not for Export EU)

Size

£735.00

SKU:: 215397
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Neuron Specific Enolase (NSE), expressed in E. coli.
Format:: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.
Extra Details:: Enolase is a metalloenzyme that catayzes the reaction between 2-phospho-D-glycerate and phosphoenolpyruvate during glycolysis. Mammalian enolase is composed of 3 subunits; alpha, beta and gamma (Neuron-specific enolase). These subunits can form homodimers or heterodimers. The alpha/gamma heterodimer and the gamma/gamma homodimer are found primarily in neurons. Applications: Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Immunocytochemistry: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.