Neurofilament Heavy, phosphorylated, non-phosphorylated (NF-200, NF200, NF-H, NEFH, N52, Neurofilament Heavy Polypeptide, Neurofilament Triplet H Protein, 200kD Neurofilament Protein, KIAA0845, NFH) (Not for Export EU)

Size

£735.00

SKU:: 215389
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Purified rat NF-H construct containing most of the tandem KSP repeats expressed and purified from E. coli.
Format:: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.
Extra Details:: Neurofilaments contain three intermediate filament proteins: light (68kD), medium (160kD) and heavy (200kD). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Applications: Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:10,000 Immunocytochemistry: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.