Nerve Growth Factor, beta, Human (Beta-NGF, NGFB)

Size

£535.00

SKU:: 215381
Molecular Weight:: 121613.5
Purity:: ~95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with 500ul PBS.
Extra Details:: Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. Beta Nerve Growth Factor, from human DNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells. Molecular Weight: ~12kD-16kD in SDS-PAGE and ~13.5kD molecular mass. Biological Activity: The ED50 of beta NGF is typically 0.2-1ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line. AA Sequence: YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEAR PVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.