Nerve Growth Factor Receptor, Fc Chimera, Human (Low-affinity Nerve Growth Factor Receptor, NGF Receptor, Gp80-LNGFR, p75 ICD, Low Affinity Neurotrophin Receptor p75NTR, p75NTR, CD271)

Size

£443.00

SKU:: 215380
Molecular Weight:: 7510050.8
Purity:: ~95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. Reconstitute with 500ul PBS.
Extra Details:: Nerve growth factor (NGF) receptor, also known as p75NTR, is a low affinity NGF receptor. It binds with equal affinity neurotrophins such as beta NGF, BDNF, NT-3 and NT-4. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The NGF receptor is a type I transmembrane glycoprotein (399aa) consisting of a signal peptide (28aa), an extracellular domain (222aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22aa) and a cytoplasmic domain (155aa). Source: Nerve Growth Factor Receptor-Fc Chimera, from human DNA sequence encoding the signal peptide and extracellular domain of human NGF receptor aa1-250 was fused to the Fc region of human IgG1 aa93-330. The chimeric protein was expressed in modified human 293 cells. Molecular Weight: ~75kD-100kD with post-translational modification and ~50.8kD unmodified. Biological Activity: The ED50 of the NGF Receptor-Fc chimera is typically 0.7-1ug/ml as measured by its ability to neutralize beta NGF mediated proliferation of the human growth- factor dependent TF-1 cell line. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice