Nerve Growth Factor (NGF, Beta NGF)

Size

£563.00

SKU:: 215378
Molecular Weight:: 2613
Purity:: ≥90% (SDS electrophoresis)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder 0.1M acetic acid. Reconstitute with 100ul sterile ddH2O or PBS.
Extra Details:: Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of ~26,500Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF Source: Nerve Growth Factor, from mouse Molecular Weight: ~26kD and the monomer ~13kD (SDS-PAGE) Biological Activity: Tested by stimulation of 8 day old chick embryo dorsal root ganglia. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice