GFAP (Glial Fibrillary Acidic Protein, Astrocyte)

Size

£754.00

SKU:: 215300
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Recombinant protein corresponding to bovine Glial Fibrillary Acidic Protein expressed in E. coli, and native bovine GFAP.
Format:: Supplied as a lyophilized powder in 5% trehalose. Reconstitute with sterile ddH2O.
Extra Details:: Glial fibrillary acidic protein (GFAP) is ~50kD intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50,000 Immunocytochemistry: 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme-linked methods Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice