TIM-4 (T-cell Immunoglobulin Mucin 4, Tim4, Timd4)

Size

£550.00

SKU:: 215175
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Tim-4-Ig, consisting of aa1-288 from mouse Tim-4, coupled with the Fc portion of mouse IgG2a.
Clone:: RMT4-54
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Tim-4 (T-cell immunoglobulin mucin 4), a transmembrane glycoprotein and member of the immunoglobulin superfamily, is exclusively expressed by antigen-presenting cells, which acts as the natural ligand of Tim-1, involved in T-cell expansion and cytokine production. Tim-4 is reported to be crucial for the phagocytosis of apoptotic cells by peritoneal macrophages, and studies have shown that interaction between Tim-1 on Th2 cells and Tim-4 on DCs, enhances Th2 cell function. The TIM-4 gene locus is associated with predisposition to asthma in both mice and humans and may be considered as a candidate disease gene for asthma. Studies have shown that Tim-4 significantly inhibits the efficient phagocytosis of apoptotic cells by PRMs (peritoneal resident Mac1+ cells), but not PEMs (peritoneal exudate Mac1+ cells) see Nakayama, M. et al. . for details. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200,10ul labels 1x10e6 cells in 100ul. Immunohistochemistry: paraffin sections Optimal dilutions to be determined by the researcher. Hybridoma: Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line. Positive Control: Mouse spleen Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice