TIM-3 (Tim3, Timd3, T-cell Immunoglobulin Mucin 3, Havcr2)

Size

£537.00

SKU:: 215171
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Tim-3-Ig, consisting of aa1-191 from mouse Tim-3, coupled with the Fc portion of mouse IgG2a.
Clone:: RMT3-23
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: T-cell immunoglobulin mucin 3, a transmembrane glycoprotein and member of the immunoglobulin superfamily, is preferentially expressed by differentiated CD4+ Th1 cells and CD8+ Tc1 (cytotoxic) cells, but not by CD4+ Th2 cells or CD8+ Tc2 cells. Tim-3 acts as a negative regulator of Th1-mediated inflammatory diseases including GVHD, type I diabetes, and EAE (experimental autoimmune encephamyelitis), promotes immunological tolerance, and is a receptor for galectin-9, shown to induce cell death of Th1 cells in vitro. Tim-3 is also involved in the regulation of macrophage activation, acts as a phagocytic receptor, and has been shown to recognize apoptotic cells via the IgV domain. Studies have shown Tim-3 significantly inhibits the efficient phagocytosis of apoptotic cells by PEMs (peritoneal exudate Mac1+ cells) ( Nakayama et al. 2009). Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200, 10ul labels 1x10e6 cells in 100ul. Immunohistochemistry: frozen/paraffin Optimal dilutions to be determined by the researcher. Hybridoma: Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice