215171-BIOTIN

TIM-3 (Tim3, Timd3, T-cell Immunoglobulin Mucin 3, Havcr2) (Biotin)

Size

£819.00

SKU:: 215171-BIOTIN
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Tim-3-Ig, consisting of aa1-191 from mouse Tim-3, coupled with the Fc portion of mouse IgG2a.
Clone:: RMT3-23
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: T-cell immunoglobulin mucin 3, a transmembrane glycoprotein and member of the immunoglobulin superfamily, is preferentially expressed by differentiated CD4+ Th1 cells and CD8+ Tc1 (cytotoxic) cells, but not by CD4+ Th2 cells or CD8+ Tc2 cells. Tim-3 acts as a negative regulator of Th1-mediated inflammatory diseases including GVHD, type I diabetes, and EAE (experimental autoimmune encephamyelitis), promotes immunological tolerance, and is a receptor for galectin-9, shown to induce cell death of Th1 cells in vitro. Tim-3 is also involved in the regulation of macrophage activation, acts as a phagocytic receptor, and has been shown to recognize apoptotic cells via the IgV domain. Studies have shown Tim-3 significantly inhibits the efficient phagocytosis of apoptotic cells by PEMs (peritoneal exudate Mac1+ cells) ( Nakayama et al. 2009). Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Immunohistochemistry: frozen/paraffin Optimal dilutions to be determined by the researcher. Hybridoma: Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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