TIM-1 (T-Cell Immunoglobin Mucin-1, KIM1, TIM1, TIMD1, HAVCR1)

Size

£550.00

SKU:: 215165
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Full-length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1.
Clone:: RMT1-10
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Tim-1 (T-cell immunoglobulin mucin 1), is a cell surface glycoprotein and member of the immunoglobulin superfamily, which was first identified as the hepatitis A virus cellular receptor (HAVCR), and is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b). Tim-1 is highly expressed by activated CD4+ T cells, and acts as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, and studies have shown that interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity. Polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and Rheumatoid arthritis. TIM-1 has been shown to promote Th2 responses, and inhibit antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies (Xiao et al. 2007). TIM-1 has been shown to reduce the severity of EAE (experimental autoimmune encephalomyelitis) and delay disease onset, in mice studies (Xiao et al. 2007). Applications: Suitable for use in Immunofluorescence, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200, 10ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice