215165-ML550
TIM-1 (T-Cell Immunoglobin Mucin-1, KIM1, TIM1, TIMD1, HAVCR1) (MaxLight 550)
Size
£819.00
- SKU:
- 215165-ML550
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Full-length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1.
- Clone:
- RMT1-10
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Tim-1 (T-cell immunoglobulin mucin 1), is a cell surface glycoprotein and member of the immunoglobulin superfamily, which was first identified as the hepatitis A virus cellular receptor (HAVCR), and is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b). Tim-1 is highly expressed by activated CD4+ T cells, and acts as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, and studies have shown that interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity. Polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and Rheumatoid arthritis. TIM-1 has been shown to promote Th2 responses, and inhibit antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies (Xiao et al. 2007). TIM-1 has been shown to reduce the severity of EAE (experimental autoimmune encephalomyelitis) and delay disease onset, in mice studies (Xiao et al. 2007). Applications: Suitable for use in Immunofluorescence, Flow Cytometry and FLISA. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice