215146
Surfactant Protein D (SPD, SFTPD)
Size
£735.00
- SKU:
- 215146
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified porcine surfactant protein D
- Clone:
- 1.7
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- Surfactant protein D (pSP-D) is a 138kD member of the collectin family of proteins. Surfactant protein D is thought to play a significant role in the innate immune response against microorganisms within the lungs and at other mucosal surfaces. SP-D is a multifunctional protein which binds selectively to saccharides and lipids on microbial surfaces promoting antimicrobial responses and regulating surfactant lipid homeostasis. SP-D expression is induced in lung surfactant in response to aerogenous bacterial infection, and upon binding to bacteria, promotes phagocytosis and antigen presentation by dendritic cells. Recent studies suggest that pSP-D could serve as a serum biomarker specific for bronchopneumonia in pigs. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1ug/ml, this product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blot: detects a band of ~138kD. Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Positive Control: Porcine lung tissue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice