215146-BIOTIN
Surfactant Protein D (SPD, SFTPD) (Biotin)
Size
£953.00
- SKU:
- 215146-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified porcine surfactant protein D
- Clone:
- 1.7
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- Surfactant protein D (pSP-D), is a 138kD member of the collectin family of proteins. Surfactant protein D is thought to play a significant role in the innate immune response against microorganisms within the lungs and at other mucosal surfaces. SP-D is a multifunctional protein which binds selectively to saccharides and lipids on microbial surfaces promoting antimicrobial responses and regulating surfactant lipid homeostasis. SP-D expression is induced in lung surfactant in response to aerogenous bacterial infection, and upon binding to bacteria, promotes phagocytosis and antigen presentation by dendritic cells. Recent studies suggest that pSP-D could serve as a serum biomarker specific for bronchopneumonia in pigs. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1ug/ml, this product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Positive Control: Porcine lung tissue Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice