SRPX1 (Sushi-Repeat-Containing Protein X-linked 1, ETX1) (HRP)

Size

£902.00

SKU:: 215138-HRP
Purity:: Purified by affinity chromatography from serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 13aa sequence from near the N-terminus of human SRPX1.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: Sushi-repeat-containing protein X-linked 1 (SRPX1), a 464 amino acid protein involved in autophagy. Applications: Suitable for use in ELISA (indirect), Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.25ug/ml, detects bands of ~75kD SK-N-SH in cell lysates Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human brain Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.