215132-ML550
Siglec 9 (Sialic Acid-binding Ig-like Lectin 9, Siglec 9, Protein FOAP-9, UNQ668, PRO1302) (MaxLight 550)
Size
£837.00
- SKU:
- 215132-ML550
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Siglec-9 fused to Fc region of human IgG.
- Clone:
- K8
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Siglec-9 (Sialic acid-binding Ig-like lectin 9), is a single pass type I membrane protein belonging to the immunoglobulin superfamily. Human Siglec-9 is broadly expressed in a range of human tissues with high expression on monocytes and low-level expression on neutrophils and subpopulations of NK, B, and T cells. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200, 10ul labels 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice