Siglec 7 (Sialic Acid-binding Ig-like Lectin 7, CD328, AIRM1)

Size

£550.00

SKU:: 215128
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Siglec-7 3T3 transfected cells.
Clone:: S7.7
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Sialic acid-binding Ig-like lectin 7, otherwise known as CD328, a putative adhesion molecule and member of the Ig superfamily, is predominantly expressed by both resting and activated natural killer (NK) cells, and also at lower levels by monocytes, granulocytes, monocyte-derived macrophages and dendritic cells, and a small population of CD8+ memory T cells. Classed as a CD33-related Siglec, Siglec-7 preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid, and acts as a novel inhibitory receptor for NK cells, mediating the inhibition of NK cell cytotoxicity, and implicated in the regulation of NK cell and T cell activation, and hematopoiesis. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:25-1:200, 10ul labels 10e6 cells Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice