215127-ML650
Siglec 5/Siglec 14 (Sialic Acid-binding Ig-like Lectin 5, CD170, CD33L2, OBBP2) (MaxLight 650)
Size
£837.00
- SKU:
- 215127-ML650
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
- Clone:
- 1A5
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, is expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Siglec-14 shares an almost identical sequence with Siglec-5 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species. Siglec-5 is highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), and mediates sialic-acid dependent binding to cells, as well as acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule, and member of the Ig superfamily, predominantly expressed in hematopoietic tissues, which has been shown to associate with the activating adapter protein DAP12. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200, 10ul labels 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice