215122-BIOTIN
Siglec 10 (Sialic Acid-binding Ig-like Lectin 10, Siglec like Protein 2, SLG2, UNQ477, PRO940) (Biotin)
Size
£837.00
- SKU:
- 215122-BIOTIN
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Siglec-10, fused with the Fc region of human IgG.
- Clone:
- 5G6
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- Siglec-10 (Sialic acid-binding Ig-like lectin 10), a putative adhesion molecule and member of the Ig superfamily, is expressed by monocytes, B cells, eosinophils, and at a higher level by a subpopulation of CD16+CD56- natural killer (NK) cells. Structurally, Siglec-10 is most similar to the CD33-related group of Siglecs, and preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid. Studies have shown that Siglec-10 acts as a substrate for VAP-1 (Vascular adhesion protein-1), a glycoprotein expressed on endothelium during inflammation, which is involved in primary amine oxidation and leucocyte trafficking, see Kive, E. et al. for details. This interaction between Siglec-10 and VAP-1, implicates Siglec-10 in endothelial lymphocyte adhesion and in the modulation of the inflammatory microenvironment. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200, 10ul labels 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice