PDGF-BB, Recombinant, Human (Platelet-Derived Growth Factor BB Homodimer, PDGFB, PDGF2, SIS)

Size

£554.00

SKU:: 215071
Molecular Weight:: 24.3
Purity:: ≥ 98% (SDS-PAGE, HPLC). Endotoxin: ≤1EU/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in 10mM acetic acid. No preservative added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant Human PDGF-BB is a 24.3kD disulfide-linked homodimer of two β chains (218 total amino acids). Recombinant protein corresponding to human PDGF BB Homodimer, expressed in E. coli Molecular Weight: ~24.3kD (218aa sequence) Amino Acid Sequence (monomer): SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT Biological Activity: PDGFB has an ED50 of 1-3ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice