N-Cadherin (CDHN, NCAD, Neural Cadherin, CD325) [KO Validated]

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£709.00

SKU:: 215041
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
Clone:: 13A9
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Neural cadherin (N-Cadherin), otherwise known as CD325 is a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry. N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. The clone studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999). The clone has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarci. The product detects a band of ~35kD-140kD in human HT-1080 and HeLa cell lysates. Applications: Suitable for use in Western Blot, Immmunohistochemistry, Immunfluorescence, Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:1000, detects a band of ~135-140kD in human HeLa cell lysates. Immunohistochemistry: Frozen and Paraffin. Paraffin requires heat treatment for antigen retrieval. KO Validated: Western blot analysis of N-Cadherin CRISPR knockout HEK293 (N-Cadherin KO) and wild type HEK293 (WT) whole cell lysates probed with (A) 215041 followed by detection with HRP conjugated Goat anti Mouse IgG (1:10,000) and (B) hFAB rhodamine anti-tubulin primary antibody and visualized on the ChemiDoc MP with (A) 40 second and (B) 30 second exposure. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice