215041-PE
N-Cadherin (CDHN, NCAD, Neural Cadherin, CD325) (PE)
Size
£960.00
- SKU:
- 215041-PE
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
- Clone:
- 13A9
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Neural cadherin (N-Cadherin), otherwise known as CD325 is a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry. N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. The clone studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999). The clone has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarci. The product detects a band of ~35kD-140kD in human HT-1080 and HeLa cell lysates. Applications: Suitable for use in Western Blot, Immmunohistochemistry, Immunfluorescence, Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen and Paraffin. Paraffin requires heat treatment for antigen retrieval. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice