MMP-2, Recombinant, Human (CLG4A, Matrix Metalloproteinase 2, Gelatinase A)

Size

£563.00

SKU:: 215034
Molecular Weight:: 62
Purity:: ≥98% (SDS-PAGE, HPLC). Endotoxin: ≤1EU/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in sodium phosphate. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
Extra Details:: MMP-2 (matrix metalloproteinase 2), otherwise known as gelatinase A, is a member of a family of secreted zinc-dependent endoproteases, which play a role in the degradation of extracellular matrix (ECM) components, including both fibrillar and non-fibrillar collagens, laminin, basement membrane glycoprotein and fibronectin. MMP-2 specifically cleaves gelatin type I, and types IV, V, VII and X collagens, and is also involved in tissue repair, inflammation, tumor invasion, vasculature angiogenesis, and the rupture of atherosclerotic plaques. Defects in the MMP2 gene are responsible for the autosomal recessive osteolysis disorder known as Torg-Winchester syndrome (TWS), or multicentric osteolysis nodulosis and arthropathy (MONA). Recombinant protein corresponding to human MMP-2 expressed in E. coli. Molecular Weight: ~62kD (552aa) containing the entire catalytic N-terminal domain and the C-terminal domain. Biological Activity: MMP-2 activity was measured by its ability to cleave a chromogenic peptide MMP-2 substrate at RT. At an MMP-2 concentration of ~2.5ug/ml, 50% cleavage was achieved at an incubation time of ~25 mins. Amino Acid Sequence: MYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPG TGVGGDSHFD DDELWTLGEG QVVRVKYGNA DGEYCKFPFL FNGKEYNSCT DTGRSDGFLW CSTTYNFEKD GKYGFCPHEA LFTMGGNAEG QPCKFPFRFQ GTSYDSCTTE GRTDGYRWCG TTEDYDRDKK YGFCPETAMS TVGGNSEGAP CVFPFTFLGN KYESCTSAGR SDGKMWCATT ANYDDDRKWG FCPDQGYSLF LVAAHEFGHA MGLEHSQDPG ALMAPIYTYT KNFRLSQDDI KGIQELYGAS PDIDLGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPMGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWIYSASTL ERGYPKPLTS LGLPPDVQRV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD AWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice