MIP-1 alpha, Recombinant, Mouse (Scya3, Mip1a, CCL3, Macrophage Inflammatory Protein-1 alpha)

Size

£492.00

SKU:: 215026
Molecular Weight:: 7.8
Purity:: ≥98% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml. For long term storage the addition of 0.1% BSA is recommended.
Extra Details:: Both MIP-1α and MIP-1β are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1α and MIP-1β exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1α selectively attracting CD8+ lymphocytes, and MIP-1β selectively attracting CD4+ lymphocytes. Additionally, MIP-1α and MIP-1β have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. Recombinant mouse MIP-1α is a 7.8kD protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. Recombinant protein corresponding to mouse MIP-1 alpha, expressed ubn E. coli. Molecular Weight: ~7.8kD (69aa) Biological Activity: Determined by its ability to chemoattract murine balb/c splenocytes using a concentration range of 10-100ng/ml. Amino Acid Sequence: APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice