215018-BIOTIN
MHC Class II Monomorphic (Biotin)
Size
£832.00
- SKU:
- 215018-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Con A activated canine peripheral blood cells.
- Clone:
- YKIX334.2
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- MHC Class II Monomorphic was classified at the First Canine Leucocyte Antigen Workshop (Cobbold et al. 1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. MHC Class II immunoprecipitates an antigen of ~32-34kD and blocks the proliferation of MHC Class II dependent responses in vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells. Applications: Suitable for use in Flow Cytometry. Immunohistochemistry, Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100. 10ul labels 1x10e6 cells in 100ul. Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Hybridoma: Y3/Ag1.2.3 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice