215015
MHC CLASS II DR (PE)
Size
£850.00
- SKU:
- 215015
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- CC108
- Format:
- Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitue in 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- MHC Class II molecules are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In cattle, this complex is referred to as the bovine leukocyte antigen (BoLA) region. There are 2 major types of MHC class IIa molecules encoded by the BoLA which are DR and DQ each composed of an alpha and beta chain. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice