215007-HRP
MHC Class I Monomorphic (HRP)
Size
£856.00
- SKU:
- 215007-HRP
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine peripheral blood mononuclear cells.
- Clone:
- ILA88
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
- Extra Details:
- Clone recognizes a monomorphic determinant within the heavy chain of bovine MHC Class I. Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This product will immunoprecipitate a band of ~45kD under reducing conditions. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice