215007-APC

MHC Class I Monomorphic (APC)

Size

£856.00

SKU:: 215007-APC
Application:: WB
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bovine peripheral blood mononuclear cells.
Clone:: ILA88
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Clone recognizes a monomorphic determinant within the heavy chain of bovine MHC Class I. Bovine MHC class I molecules are expressed at varying levels on most nucleated cells, with exception of neural cells. This product will immunoprecipitate a band of ~45kD under reducing conditions. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.