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215000

MCP-1, Recombinant, Mouse (CCL2, JE, Je, Mcp1, Scya2)

Size

£544.00

SKU:
215000
Molecular Weight:
13.8
Purity:
≥98% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug (<1EU/ug)
Storage Conditions:
-20°C
Supplier:
United States Biological
Format:
Supplied as a lyophilized powder. No preservative added. Reconstitute in with sterile ddH2O to 0.1-1mg/ml.
Extra Details:
The MCP proteins are members of the CC chemokine family that signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. The MCP family cross-reacts across species. Recombinant mouse JE is a 13.8 kDa protein containing 125 amino acid residues, including the four highly conserved cysteine residues present in the CC chemokines. Recombinant protein corresponding to mouse MCP-1, expressed in E. coli. Molecular Weight: ~13.8kD (125aa) Amino Acid Sequence: QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN Biological Activity: Determined by its ability to chemoattract Balb/C mouse spleen MNCs using a concentration range of 1-20ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice