M-CSF, Recombinant, Mouse (Macrophage Colony-stimulating Factor, Csfm)

Size

£544.00

SKU:: 214990
Molecular Weight:: 36.4
Purity:: ≥98% (SDS-PAGE, HPLC). Endotoxin: < 0.1ng/ug (<1EU/ug)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: M-CSF is a potent hematopoietic factor produced by a variety of cells, including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival for blood monocytes, tissue macrophages, and their respective progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness and fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Human M-CSF is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant mosue M-CSF is a 36.4kD homodimeric protein consisting of two 156 amino acid polypeptide subunits. Recombinant protein corresponding to mouse M-CSF expressed in E. coli Molecular Weight: ~36.4kD (156aa) Biological Activity: The ED50 was determined by the dose dependent stimulation of the proliferation of murine M-NSF-60 cells is <1ng/ml, corresponding to a specific activity of >1x10e6units/mg. Amino Acid Sequence (monomer): MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice