Lipoprotein Lipase (LDL)

Size

£709.00

SKU:: 214978
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified bovine milk lipoprotein lipase. Epitope: aa380-410
Clone:: 5D2
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Lipoprotein lipase (LPL) is a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues. A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions. 214978 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. 214978 has been shown to inhibit the activity of human LPL (Chang et al. 1998), and is widely used in human studies. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:5000 in an indirect ELISA, or as the capture antibody in a sandwich ELISA with 214981 or 214979 as the detection reagent. Western Blot: detects a band of ~53kD using partially purified LPL from postheparin plasma. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice