214947
Interleukin-4, Recombinant, Human (IL-4, B-Cell Stimulatory Factor 1)
Size
£358.00
- SKU:
- 214947
- Molecular Weight:
- 15.113
- Purity:
- ≥98% (SDS-PAGE, HPLC). Endotoxin: ≤1EU/ug
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml. For extended storage it is recommended to further dilute in a buffer containing 0.1% BSA.
- Extra Details:
- This product, recombinant human interleukin-4, also known as B-cell stimulatory factor 1, is a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. Excessive secretion of IL-4 by Th2 helper cells, has been linked with allergy and elevated IgE levels. Recombinant protein corresponding to human Interleukin-4 expressed in E. coli Molecular Weight: ~15.1kD (130aa) Biological Activity: The ED50 as determined by the dose dependent stimulation of human TF1 cells is <0.2ng/ml, corresponding to a specific activity of >5x10e6units/mg. Amino Acid Sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice