Interleukin-3, Recombinant, Human (IL-3)

Size

£544.00

SKU:: 214946
Molecular Weight:: 15
Purity:: ≥98% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Supports the formation of multilineage colonies in the early development of multipotent haematopoietic progenitor cells. It has been shown to induce colony formation of macrophages, neutrophils, mast cells and megakaryocytes from agar-suspended bone marrow cells. Recombinant protein corresponding to human Interleukin-3 expressed in E. coli Molecular Weight: ~15kD (133aa) Amino Acid Sequence: APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1ng/ml, corresponding to a specific activity of ≥ 1x10e7 units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.