Interleukin-12, p70, Recombinant, Human (IL-12, NKSF1, NKSF2)

Size

£544.00

SKU:: 214930
Molecular Weight:: 75
Purity:: ≥98% (SDS-PAGE, HPLC). Endotoxin: <0.1ng/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 50ul sterile PBS. For extended storage, the addition of 0.1% BSA is recommended. Do not vortex.
Extra Details:: IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-γ-producing Th1 cells. Recombinant Human IL-12 is a 75kD heterodimeric glycoprotein consisting of disulfide-linked 35kD (p35) and 40kD (p40) subunits (503 total amino acid residues). Recombinant protein corresponding to human Interleukin-12 expressed in CHO cells. Molecular Weight: ~75kD (503aa) Amino Acid Sequence: p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS Biological Activity: Assay #1: Determined by its ability to induce IFN-γ production from NK cells co-stimulated with IL-18. The expected ED50 is ≤ 1ng/ml, corresponding to a specific activity of ≥ 1x10e6 units/mg. Assay #2: Determined by its ability to increase IFN-γ production by anti-TCR mAb-stimulated PBMCs. The expected ED50 for this effect is 4-8ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice