214927
Interleukin-1 beta, Recombinant, Rat (IL-1 beta)
Size
£554.00
- SKU:
- 214927
- Molecular Weight:
- 17.4
- Purity:
- ~98% (SDS-PAGE, HPLC). Endotoxin: ≤0.1ng/ug
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder in 10mM sodium acetate, pH 6.5. No preservative added. Reconstitute with 100ul sterile ddH2O.
- Extra Details:
- Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Recombinant protein corresponding to rat Interleukin-1 beta expressed in E. coli. Molecular Weight: ~17.4kD (153aa) Biological Activity: >1x10e7units/mg as determined by dose-dependent stimulation of proliferation of murine D10S cells. Amino Acid Sequence: MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice