HIF1 Alpha (Hypoxia-Inducible Factor 1 alpha)

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£709.00

SKU:: 214885
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human HIF-1 Alpha.
Clone:: Halpha111a
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Hypoxia-inducible factor 1-alpha otherwise known as HIF1 alpha (HIF1A) or ARNT interacting protein, is a widely expressed bHLH-PAS transcription factor, which acts as a critical regulatory protein in the host response to hypoxia (Kubis et al. 2005). HIF1A and HIF1B form the highly-conserved heterodimeric HIF-1 transcriptional complex (Carmeliet et al. 1998). During hypoxic conditions, HIF1A accumulates in the nucleus and activates the transcription of many genes encoding proteins involved in the production of oxygen delivery and metabolic adaptation, such as vascular endothelial growth factor and glucose transporters. Under normal oxygen conditions, HIF1A is targeted by HIF prolyl-hydroxylases, followed by rapid protease degradation, which is inhibited during hypoxia (Hirsilä et al. 2003). Overexpression of HIF1A occurs in many common human cancers, including pancreatic, bladder and renal carcinomas (Shibaji et al. 2003). Mouse anti Human HIF1alpna, has been successfully used for the identifcation of HIF1alpha in human samples by both immunohistochemistry and Western blotting. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:25. Requires antigen retrieval using heat treatment using Tris/EDTA buffer pH 9.0 prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control Tissue: Human tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice