214860
Glutamate Decarboxylase 2 (GAD65/GAD2)
Size
£709.00
- SKU:
- 214860
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- KLH conjugated synthetic peptide sequence PGSGFWSFGSEDGSGDSEN corresponding to amino acids 4-22 within the N-terminal region of human GAD65.
- Clone:
- N-GAD65
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- GAD65 catalyzes the decarboxylation of glutamate to GABA, the major inhibitory neurotransmitter in the central nervous system. GAD65 is the 65kD isoform of GAD, encoded by the GAD2 gene, which is predominantly expressed by nerve termini, as oppose to the 67kD isoform (GAD67), which is predominantly found in the cell body, and is encoded by the GAD1 gene. Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, including Graves’ disease and Stiff Man Syndrome (SMS), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, and those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles. Applications: Suitable for use in RIA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0/AG14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice