GFAP (Glial Fibrillary Acidic Protein)

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£634.00

SKU:: 214855
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bovine spinal cord homogenate.
Clone:: 2E1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Glial fibrillary acidic protein, or GFAP, is a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood-brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell. Defects in GFAP are a cause of Alexander disease, a rare disorder of the central nervous system affecting mostly males. It is a progressive leukoencephalopathy resulting in mental and physical retardation, dementia, seizures and early death. May be used in conjunction with 214854 and G2037-05D for increased sensitivity when used in immunohistology. Applications: Suitable for use in RIA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:200-1:2000 Immunohistochemistry: Frozen and paraffin sections. Requires protein digestion pretreatment of paraffin sections e.g. trypsin or pronase. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice